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Abstract: Objective: To investigate the association of high-risk human papilloma virus (HR-HPV) and other risk factors with ocular surface squamous cell neoplasia (OSSN). Materials and methods: We obtained DNA from 22 fresh frozen OSSN tissues and 22 pterygia as controls, we used a broad-spectrum HPV DNA amplification short PCR fragment to identify HPV infection in all specimens and then genotyped HPV by a reverse hybridization line probe assay. We also obtained demographic, sun exposure, and tobacco consumption information. Results: HR-HPV frequency was 40.9% in the OSSN group and 4.5% in the pterygia group (p=0.009). After covariate adjustment, OSSN was associated with HR-HPV (OR=16.3, 95%CI=1.2,218.1, p=0.03) and sunburn (OR=10.8, 95%CI=1.8,86.0, p=0.02). Conclusions: Ocular surface squamous cell neoplasia is a multifactorial disease. The strong association between HR-HPV and OSSN, suggests that HR-HPV could play an etiological role in OSSN development.
Resumen: Objetivo: Investigar la asociación del virus del papiloma humano de alto riesgo (VPH-AR), así como de otros factores, con neoplasia escamosa de la superficie ocular (NESO). Material y métodos: Se obtuvieron 22 especímenes de tejido fresco de NESO y 22 de pterigión como controles; se utilizó una técnica molecular altamente sensible para identificar la infección por VPH en todos los especímenes, así como la genotipificación del VPH. También se obtuvo información demográfica sobre exposición a la luz solar y tabaquismo. Resultados: La frecuencia de infección por VPH-AR fue de 40.9% en el grupo de NESO y de 4.5% en el grupo control (p=0.009). Después de ajustar por covariables, NESO se asoció con el VPH-AR (OR=16.3, IC95%=1.2,218.1, p=0.03) y el eritema solar (OR=10.8, IC95%=1.8,86.0, p=0.02). Conclusiones: La neoplasia escamosa de superficie ocular en una neoplasia multifactorial. Los presentes resultados sugieren que el VPH-AR podría tener un papel etiológico en el desarrollo de NESO.
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Abstract Introduction Squamous cell carcinoma is the most common laryngeal neoplasm and accounts for approximately 95% of all malignant neoplams of the larynx. However, various benign and malignant tumors and inflammatory diseases may affect the larynx. Objective The purpose of this study is to analyze the clinical and imaging findings of non-squamous cell neoplasms and inflammatory diseases of the larynx. Methods This retrospective study was conducted in 18 patients who were diagnosed with non-squamous cell carcinoma lesions of larynx at our institution between 2007-2017. Clinical symptoms, examination findings, imaging characteristics, histopathologic diagnosis and treatment modalities were analyzed. Results There were 9 malignant lesions (2 chondrosarcoma, 1 neuroendocrine tumor-atipical carcinoid, 1 Natural Killer/T-cell lymphoma, 1 diffuse large B-cell lymphoma, 3 plasmocytoma-multiple myeloma involvement, 1 adenocarcinoma metastasis), 3 benign neoplasms (chondroma, paraganglioma, lipoma), 2 tumor-like lesions (Brown tumor and inflammatory myofibroblastic tumor), 3 inflammatory lesions (Wegener granulomatosis, Behçet's disease and tuberculosis involvements), and 1 vascular malformation. The most common presenting symptom was hoarseness (66.6%). Paraganglioma was seen as hypervascular lesion on computed tomography and magnetic resonance imaging and showed intense tracer uptake on 68Gallium-DOTA-peptide PET/CT. Chondroid matrix calcifications were detected in chondroma and chondrosarcoma-grade 1. In patients with vascular malformation and lipoma, the typical imaging findings made it possible to diagnose. Conclusion Imaging studies may provide clues for diagnosis of non-squamous cell laryngeal lesions. Clinical and imaging findings and previous clinical history should be evaluated together in clinical management of laryngeal lesions.
Resumo Introdução O carcinoma de células escamosas é a neoplasia laríngea mais comum, representa aproximadamente 95% de todas as neoplasias malignas da laringe. No entanto, vários outros tumores benignos e malignos, e doenças inflamatórias, podem afetar a laringe. Objetivo O objetivo deste estudo é analisar os achados clínicos e de imagem de neoplasias de células não-escamosas e de doenças inflamatórias da laringe. Método Este estudo retrospectivo foi feito com 18 pacientes diagnosticados com lesões de carcinoma de células não escamosas da laringe em nossa instituição, entre 2007-2017. Foram analisados os sintomas clínicos, achados dos exames, características de imagens, diagnóstico histopatológico e modalidades de tratamento. Resultados Foram identificados 9 casos com lesão maligna (2 condrossarcomas, 1 tumor carcinoide neuroendócrino atípico, 1 linfoma de células T/NK, 1 linfoma difuso de grandes células B, 3 plasmocitomas com envolvimento de mieloma múltiplo, 1 metástase de adenocarcinoma, 3 neoplasias benignas (condroma, paraganglioma, lipoma), 2 lesões tumor like (tumor de Brown e tumor miofibroblástico inflamatório), 3 lesões inflamatórias (granulomatose de Wegener, doença de Behçet e tuberculose) e 1 malformação vascular. O sintoma mais comum foi a rouquidão (66,6%). O paraganglioma foi visto como lesão hipervascular na tomografia computadorizada e na ressonância magnética e mostrou intensa captação do traçador na PET/TC com 68Gálio-DOTA. Calcificações de matriz condroide foram detectadas no condroma e condrossarcoma grau 1. Em pacientes com malformação vascular e lipoma, os achados típicos de imagem tornaram possível o diagnóstico. Conclusão Estudos de imagem podem fornecer pistas para o diagnóstico de lesões laríngeas de células não escamosas. Achados clínicos e de imagem e histórico clínico prévio devem ser avaliados em conjunto no manejo clínico das lesões laríngeas.
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Humans , Laryngeal Neoplasms , Larynx , Bone Neoplasms , Carcinoma, Squamous Cell , Retrospective Studies , Positron Emission Tomography Computed TomographyABSTRACT
Resumen El carcinoma escamocelular (CEC) tiene un comportamiento biológico más agresivo cuando exhibe factores de riesgo. En este grupo, se comprometen los ganglios linfáticos hasta en 21% de los casos lo que disminuye la supervivenciaa5años. El examen clínico de las cadenas ganglionares puede tener una tasa de falsos negativos cercana a 39%, determinando la necesidad de una herramienta que permita evaluar el compromiso ganglionar de un modo más preciso. La biopsia del ganglio centinela ha sido documentada en la estadificación del CEC de cabeza y cuello, y el CEC de origen anogenital, pero en estas series de casos hay un escaso número de pacientes correspondientes a CEC cutáneo del tronco y las extremidades. El objetivo de esta revisión es describir las indicaciones y la plausibilidad de estadificar la extensión ganglionar usando la biopsia de ganglio centinela en pacientes con CEC cutáneo del tronco y extremidades. © 2017 Instituto Nacional de Cancerologia. Publicado por Elsevier España, S.L.U. Todos los derechos reservados.
Abstract Squamous Cell Carcinoma (SCC) is associated with a more aggressive biological behaviour in the presence of risk factors. High risk SCC may present with lymph node metastasis in 21% of patients, with the consequent reduction in overall survival. The physical examination lymph nodes can have a false-negative rate between 15% and 39%, thus requiring the need to find a new diagnostic tool that allows a more precise evaluation of lymph node involvement. Sentinel lymph node biopsy has been reported in case series of patients with head and neck SCC, and genital SCC, but there are few case reports about skin SCC of the trunk and extremities. The aim of this review is to describe the indications and feasibility of using sentinel lymph node biopsy to assess lymph node status in patients with skin SCC of trunk and limbs. © 2017 Instituto Nacional de Cancerologia. Published by Elsevier Espana, S.L.U. All rights reserved.
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Humans , Skin , Carcinoma, Squamous Cell , Sentinel Lymph Node Biopsy , Extremities , Sentinel Lymph Node , Role , Risk Factors , Medical Oncology , Neoplasm MetastasisABSTRACT
BACKGROUND: Nonmelanoma skin cancer is the most common form of cancer in humans and also the malignant disease that is increasingly common among kidney transplant recipients. OBJECTIVE: To determine the epidemiological characteristics of renal transplant recipients with nonmelanoma skin cancer seen at a referral transplantation center. METHODS: Cross-sectional descriptive study with renal transplant recipients presenting nonmelanoma skin cancer, treated at a transplantation referral center between 08/01/2004 and 08/31/2009. Analyzed variables were: gender, age, skin phototype, occupational and recreational sun exposure, use of photoprotection, personal and family history of non-melanoma skin cancer, clinical type and location, time between transplantation and the appearance of the first nonmelanoma skin cancer, occurrence of viral warts, timing of transplantation, type of donor, cause of kidney failure, previous transplants, comorbidities, pre-transplant dialysis, type and duration of dialysis. RESULTS: 64 subjects were included. Males - 71.9%; low skin phototypes (up to Fitzpatrick III) - 89%; mean age - 57.0 years - and mean age at transplant - 47.3 years; sun exposure - 67.2% occupational - and 64.1% recreational; photoprotection - 78.2% (although only 34.4% in a regular manner); squamous cell carcinoma - 67.2%; squamous cell carcinoma/basal cell carcinoma ratio - 2:1; personal history of nonmelanoma skin cancer - 25% - and family history - 10.9%; location at photoexposed area - 98.4%; average latency time between transplantation and first nonmelanoma skin cancer appearance - 78.3 months; viral warts (HPV) after transplant - 53.1%; average timing of transplantation - 115.5 months; living donor - 64.1%; triple regimen (antirejection) - 73.2%; comorbidities - 92.2%; pre-transplant dialysis - 98.4%; hemodialysis - 71.7%; average duration of dialysis - 39.1 months; previous transplants - 3.1%; hypertension as cause of renal failure ...
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Carcinoma, Basal Cell/etiology , Carcinoma, Squamous Cell/etiology , Kidney Transplantation/adverse effects , Skin Neoplasms/etiology , Brazil , Cross-Sectional Studies , Carcinoma, Basal Cell/epidemiology , Carcinoma, Squamous Cell/epidemiology , Immunocompromised Host , Immunosuppression Therapy/adverse effects , Immunosuppressive Agents/adverse effects , Risk Assessment , Risk Factors , Skin Neoplasms/epidemiology , Time FactorsABSTRACT
Objective To construct a small interfering RNA (siRNA) vector targeting ΔNp63α and investigate ΔNp63α gene interference on the proliferation and apoptosis of human esophageal squamous carcinoma Eca109 cell line. Methods Adeno-associated virus (AAV)-ΔNp63αshRNA driven by H1 promoter was constructed and was used to infect Eca109 cells. AAV-Null and normal cell lines were utilized in the control group and blank control group, respectively. The influence of siRNA interference of ΔNp6α expression on the growth, proliferation, tumorigenic efficiency and apoptosis of Eca109 cells were analyzed in vitro and in vivo. Results Compared with the two control groups, the specific siRNA targeting ΔNp63α gene significantly down-regulated the expression of ΔNp63α protein levels in Eca109 cells (all P<0.05). The growth of Eca109 cells infected with AAV-ΔNp63αshRNA was significantly lower than those in the two control groups (all P<0.05). Cell cycle analysis showed the proliferation index (PI) of AAV-ΔNp63αshRNA infected cell line was significantly lower compared with the two control groups (all P<0.01). In vivo experiment exhibited that AAV-ΔNp63αshRNA infected cells resulted in a lower tumor weight in nude mice compared with the cells in the two control groups (all P<0.05). In addition, the apoptosis index (AI) of AAV-ΔNp63αshRNA infected cells were significantly higher than those of the other cell lines (P<0.05). Conclusion AAV- mediated expression of shRNA can significantly reduce ΔNp63α expression in Eca109 cells, slowing down the proliferation, promoting the apoptosis, and subsequently inhibiting the growth of tumor.
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Objective: To study the influence of Wnt5a gene silence on the proliferation, migration and invasion of lung squamous carcinoma cells. Methods: A recombinant plasmid pH1-siRNAWnt5a- was constructed and used to deliver small interference RNA (siRNA) targeting Wnt5a in SK-MES-1 cells; the transfected cells were screened to establish a stable transgenic cell line. MTT, cell cycle and Transwell assays were employed to evaluate the effect of Wnt5a gene silence on the proliferation, migration and invasion of lung squamous carcinoma cells. Results: Western blotting assay revealed that Wnt5a was lowly expressed in SK-MES-1Wnt5a- (13.6%). The proliferation index (PI) of transgenic cell line was slightly lower than that of the control cell line ([28.3±3.8] % vs [30.5±5.2]%). The migration and invasion capabilities of SK MES-1Wnt5a- cells were (47.3±9.2)% and (39.7±11.7)% of the control cells, respectively. Conclusion: Low Wnt5a expression can significantly inhibit the migration and invasion capabilities of SK-MES-1 cells, indicating that WntSa might be a potential target for gene therapy of lung squamous carcinoma.
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OBJECTIVE: We aim at determining the prognostic value of squamous differentiation in patients with transitional cell carcinoma (TCC) of the bladder that were treated with radical cystectomy. MATERIALS AND METHODS: From January 1993 to January 2005, we retrospectively selected 113 patients. Correlations among squamous differentiation with other clinical and pathological features were assessed by both chi-square and Fisher tests. The Kaplan-Meier method was used to evaluate survival curves and statistical significance was determined by the log-rank test. Multivariate analysis was performed through a Cox proportional hazards regression model. RESULTS: Squamous differentiation was observed in 25 (22.1 percent) of the 113 patients. This finding was significantly related only to the pathological stage. Mean follow-up after cystectomy was 31.7 ± 28.5 months. Disease recurrence occurred in 16 (64 percent) and 30 (34 percent) patients with and without squamous differentiation (log-rank test, p = 0.001), and mortality occurred in 10 (40 percent) and 14 (16 percent) of the patients with and without squamous differentiation respectively. Univariate analysis revealed that pathological stage, squamous differentiation, tumor size and lymph node involvement were significant predictors of cancer-specific survival. However, only squamous differentiation and tumor size were independent prognostic variables on multivariate analysis. CONCLUSIONS: Squamous differentiation was an independent prognostic factor for cancer specific survival in patients with bladder cancer treated with radical cystectomy. Further studies with a larger number of patients are necessary to confirm these results.
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Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell/surgery , Carcinoma, Transitional Cell/surgery , Cystectomy/methods , Urinary Bladder Neoplasms/surgery , Carcinoma, Squamous Cell/mortality , Carcinoma, Squamous Cell/pathology , Carcinoma, Transitional Cell/mortality , Carcinoma, Transitional Cell/pathology , Epidemiologic Methods , Neoplasm Staging , Prognosis , Survival Analysis , Treatment Outcome , Urinary Bladder Neoplasms/mortality , Urinary Bladder Neoplasms/pathologyABSTRACT
Objective:To study the role of basic fibroblast growth f ac tor (bFGF) on angiogenesis in oral squamous cell carcinoma(OSCC).Methods :Expression of bFGF and CD34 in 42 cases of OSCC(14 cases with lymph no de metastasis) and 10 cases of normal oral mucosa(NOM) was detected by immunohis tochemistry. Microvessel density(MVD) in OSCC was assessed with microscope. Results:bFGF was detected in 29(69.05%) cases of OSCC and in 3(33.3 3%) of NOM. MVD in OSCC and NOM was 53.50?9.59 and 23.30?7.33(P
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Objective:To investigate therapeutic effect and toxici ti es of neoadjuvant chemotherapy(NACT) with Carboplatin(CBP) and 5-Fluorouracil (5-FU) for the patients with oral squamous cell carcinoma(OSCC)by chronomodul ated administration. Methods:73 patients with T3 and T4 OSCC, ad mitted from March 2002 to April 2004, were randomly divided into two groups,37 c ases were in chrono-chemotherapy group (groupⅠ) and 36 cases in routine-chemo therapy group (group Ⅱ). Therapeutic effects and side effects between tw o groups were compared.Results:After two NACT courses, the effec tive rate in group Ⅰ and group Ⅱ was 75.68% and 52.78%respectively (P
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Objective: To observe HLA DR expression in different s tages of oral squamous cell carcinoma (OSCC) and to study the clinic significan ce of the abnormal HLA DR expression in primary OSCC. Method: HLA DR expression was detected by immunohistochemistry method in 26 cas es of histologically normal oral epithelia, 8 leukoplakia, 32 primary focuses an d 12 metastasis focuses of OSCC. Results: HLA DR express ion in primary OSCC focuses was significantly higher than that in normal epithel ium( P 0.05). Conclusion: Although HLA DR expression is frequently obs erved in the development of OSCCs, it can not be regarded as one of the indepen dent prognosis factors.
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Objective:To determine the effects of angiogenesis in c ervical lymph node metastasis and progression of squamous cell carcinomas of gin giva (GSCC), and the distribution and expression of vascular endothelial growth factor (VEGF) in GSCC.Methods: Paraffin sections of surg ically obtained GSCC samples from 42 patients were stained with CD34 monoclon al antibody by immunohistochemical S P method to demonstrate blood vessels. Exp ression of VEGF was tested with the S P method, and the microvessel density (M VD) was determined according to the percent of the neoplastic cells showing VEG F immunoreactivity and the degree of staining.Results: The MVD in GSCC was significantly higher than that in normal gingival tissue( P 0.05). MVD in VEGF positive samples of GSCC was sig nificantly higher than that in VEGF negative ones ( P
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Objective: To investigate the correlation between neck lymph node metastasis and focal adhesion kinases(FAK) expression in oral squamous cell carcinoma(OSCC). Methods: Immunohistochemistry LsAB technique was used to observe the expression of FAK in primary tumour and nodal metastasis of OSCC in 80 cases, correlation between the expression of FAK in OSCC and neck lymph node metastasis was analyzed statistically. Results: The results demonstrated that FAK was expressed in all 80 cases, the expression of FAK in the peripheral area of OSCC was stronger than that in central area (P
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Objective:To study the significancy of P27 Kip1 expression, cell apoptosis and cell cycle distribution in the evaluation of sensitivity in oral cancer chemotherapy. Methods:40 cases of oral tumor were treated with MTX,CDDP and PYM by subcutaneous implantable drug pump.Operation was performed 2~4 weeks after the induction chemotherapy. Cell cycle distribution, apoptosis and P27 Kip1 protein expression in the removed tumor tissue were detected by fllowcytometry and indirect fluoressent assay pre-and post-treatment.Results:(1)After induction chemotherapy S,G_2M and PI ration in cell cycle distribution decreased,G_0/G_1, AP and FI_ p27 increased(P
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Objective: To study the role of transforming growth fa ctor ?1(TGF-?1) in the procession of human oral squamous cell carcinoma. Methods: Immunohistiochemical techinic was used to determine o ncogene c-myc,c-erbB2,TGF-?1 and EGFR protein expression in oral squamou s cell carcinoma cell line TSCCa and metastasis carcinoma cell line GNM treated by different levels of TGF-?1. Results: TGF-?1 showed different effects on the two cell lines. TGF-?1 significantly increased the expression of C-myc,CerbB2 and slightly enhanced TGF-?1 and EGFR protein expression in GNM cells, while TGF-?1 decreased the expression of C-myc,C erbB2,TGF-?1 and EGFR in TSCCa cells; moreover, the changes of protein ex pression of C-myc,CerbB2 were dose-dependent on TGF-?1. Conclusi on: TGF-?1 have different role in the oral squamous cell carcinoma cell lines.
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Objective: To observe the expression of proteins Rb and P 53 in leukoplakia (LK) and squamous cell carcinoma (SCC) of oral muco sa. Methods: The expression of proteins Rb and P 53 was determined by immunohistochemical S-P method. Paraffin sections of 33 s amples of SCC and 20 of LK were immunohistochemically stained and analyzed. Results: (1)The expression of Rb in OLK, OSCC was not si gnificantly different from that in normal oral mucosa ;(2)the overexpression of mutant P 53 were found in OLK and its expression was increasing with dysp hasia degree (P
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Objective:To study the relation among nm23-H 1 expression,DNA contents and lymph node metastases of head and neck squamous cell carcinoma(HNSCC).Methods:Fresh specimens from 30 previously untreated patients with HNSCC were examined for the determination of nm23-H 1 expression with immunohistochemistry, Aneuploid and S-phase fraction(SPF) with flow cytometry(FCM).The association among these parameters with lymph node metastases was studied.Results: nm23-H 1 expression was found in 18 cases (60%).and aneuploid in 20 (66.7%).Correlation was found between lymph node metastasis and nm23-H 1 protein expression (P
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Objective:To assess regional occult metastases on clinical negative neck node [cN0] in patients with buccal mucosal squamous cell carcinoma. Methods:69 patients (31males and 38 females ) with cN0 buccal mucosa squamous cell carcinoma who underwent a lymph node dissection (at the Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital, Nanjing University Medical School) between 1992 and 2004, were retrospectively reviewed. The patients ranged in age from 31 to 79 years with a mean age of 58.2 years. All patients received radical neck dissection(RND), functional neck dissection (FND) or supraomohyoid neck dissection (SOND). Results:The incidence of occult metastases for patients with cN0 buccal mucosa squamous cell carcinoma was 14.49% (10/69).The distribution of the positive nodes was as follows: Level I,10.14%(7/69); Level II, 5.80% (4/69); Level III, 2.90%(2/69). The occult metastasis rate for patients with T1, T2, T3 and T4 was 9.52% (2/21), 15.38%(6/39), 25.00%(1/4), and 20.00%(1/5), respectively. In patients with well differentiated carcinoma,the occult metastasis rate was 14.89%(7/47); moderate differentiated,10.00%(2/20); and poorly differentiated,50.00%(1/2). Conclusion:The assessment of regional occult metastases for patients with cN0 buccal mucosa squamous cell carcinoma may play an important role in deciding treatment approaches of neck.
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Objective:To investigate the expression of Hpa and its correlation with the microvessel density(MVD) in oral squamous-cell carcinoma(OSCC), thereby learning the function of Hpa in the tumor angiogenesis,invasion and lymph node metastasis.Methods:The expression of Hpa was studied in 48 patients with OSCCs, 15 patients with leukoplakia and 10 normal person with healthy oral mucosa by conventional immunohistochemistry and in situ hybridization. MVD was assessed with anti-CD31 antibody. Results: Positive expression ratios of Hpa and Hpa mRNA in leukoplakia were significantly higher than those in normal oral mucosa, while were significantly lower than in OSCC(P
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Objective:To analyze the postoperative recurrentive factors of oral squamous cell carcinoma.Methods: 673 patients of primary oral squamous cell carcinoma were involved. The focus location, growth type, T stage, lymphonodus metastasis, treatment, pathological grading and resection mode of mandible were evaluated to determine the prognosis of patients.Results:The postoperative recurrence rate was relevant to the focus location, growth type, T stage, lymphonodus metastasis and pathological grading, while the treatment and resection mode of mandible had no effects on the recurrence rate. Conclusion: It's of great importance to estimate all kinds of clinicopathologic factors and perform preventive treatments during the clinical work to promote the operative cure rate and survival rate of patients with oral squamous cell carcinoma.
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Objective: To investigate the expression of P16 protein in oral verrucous carcinoma (VC) and oral squamous cell carcinoma (SCC) and to reveal its role in the occurrence and development of VC . Methods: Using streptavidin/peroxidase(SP) immunohistochemical technique(IHC) the expression of P16 protein in 41 samples, including 8 of normal mucosa (NM),13 VC,10 well differentiated squamous cell carcinoma (wdSCC),10 poorly differentiated squamous cell carcinoma (pdSCC) was studied.The average intensity score (IS) of immunohistochemical staining of the samples was calculated. Results: Weakly positive of P16 protein was obsereved in the 8 cases of NM. Positive expression was found in 10 of the 13 VC cases,8 of the 10 wdSCC and 6 of the 10 pdSCC.The IS in NM,VC, wd SCC and pdSCC was 0.4375?0.0498,1.5846?0.2681,0.9900?0.1894 and 0.8800?0.2590 respectively.The mean intensity of P16 protein in VC was higher than in wdSCC and pdSCC(P